Biocompatible label-free detection of carbon black particles by femtosecond pulsed laser microscopy

Hannelore Bové, Christian Steuwe, Eduard Fron, Eli Slenders, Jan DHaen, Yasuhiko Fujita, Hiroshi Uji-i, Martin vandeVen, Maarten Roeffaers, Marcel Ameloot (see publication in Journal )


Although adverse health effects of carbon black (CB) exposure are generally accepted, a direct, label-free approach for detecting CB particles in fluids and at the cellular level is still lacking. Here, we report nonincandescence related white-light (WL) generation by dry and suspended carbon black particles under illumination with femtosecond (fs) pulsed near-infrared light as a powerful tool for the detection of these carbonaceous materials. This observation is done for four different CB species with diameters ranging from 13 to 500 nm, suggesting this WL emission under fs near-infrared illumination is a general property of CB particles. As the emitted radiation spreads over the whole visible spectrum, detection is straightforward and flexible. The unique property of the described WL emission allows optical detection and unequivocal localization of CB particles in fluids and in cellular environments while simultaneously...