Dynamics of the phospholipid shell of microbubbles: a fluorescence photoselection and spectral phasor approach
Eli Slenders, Senne Seneca, Sumit Kumar Pramanik, Nick Smisdom, Peter Adriaensens, Anitha Ethirajan, Marcel Ameloot (see publication in Journal )Abstract
The lipid organization of microbubbles is important in many applications. By monitoring the photoselection and emission spectrum of the fluorescent probe Laurdan in perfluorobutane gas-filled DPPC microbubbles with a two-photon laser scanning microscope, we observed a transition to a more rigid lipid organization in 30 minutes to several hours.