Dynamics of the phospholipid shell of microbubbles: a fluorescence photoselection and spectral phasor approach

Eli Slenders, Senne Seneca, Sumit Kumar Pramanik, Nick Smisdom, Peter Adriaensens, Anitha Ethirajan, Marcel Ameloot (see publication in Journal )


The lipid organization of microbubbles is important in many applications. By monitoring the photoselection and emission spectrum of the fluorescent probe Laurdan in perfluorobutane gas-filled DPPC microbubbles with a two-photon laser scanning microscope, we observed a transition to a more rigid lipid organization in 30 minutes to several hours.